Further evidence for egg transformation in Nicotiana
نویسندگان
چکیده
منابع مشابه
High-Efficiency Agrobacterium-Mediated Transformation of Tobacco (Nicotiana tabacum)
To improve Agrobacterium-mediated transformation of tobacco, factors influencing gene delivery, including genotype of the plant, bacterial strain, and Agrobacterium transformation procedure, were tested via direct somatic embryogenesis. Leaf tissue of three different tobacco genotypes (Nicotiana tabacum L. cvs. Samsun, and Xanthi, and N. benthamiana) we...
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Sterile root cultures from Nicotiana tabacum were grown with H(3)-thymidine added to the medium for various intervals. Incorporation of the labeled nucleoside into nuclear DNA occurred in a fraction of the nuclei which increased with time. In addition, the cytoplasm of all cells incorporated enough tritium to be readily detected by autoradiography. The tritium was not removed by hydrolysis in 1...
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ژورنال
عنوان ژورنال: Heredity
سال: 1980
ISSN: 0018-067X,1365-2540
DOI: 10.1038/hdy.1980.47